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rnascope probe ctip2, c1 and c2  (Advanced Cell Diagnostics Inc)


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    Advanced Cell Diagnostics Inc rnascope probe ctip2, c1 and c2
    (A) Violin plot demonstrating the expression levels of three representative DEGs by cell type at P0.5. Rora and Nrxn3 are downregulated whereas Kirrel3 is upregulated. (B and C) Representative RNA in situ hybridization (ISH) images for Rora (B) and Kirrel3 and Nrxn3 (C) confirm snRNA-seq findings in control and Foxp1 cKO mice at P0.5. <t>Ctip2</t> was used to label L5–L6 cortical neurons. (D) The total numbers of pixels in each of the 4 bins were measured in the Ctip2 -expressing layer and normalized to the total nuclei in their respective bins. (E–G) Boxplots illustrate significant downregulation of Rora (E) and Nrxn3 (F) and upregulation of Kirrel3 (G) in the L5–L6 cortical layers of Foxp1 cKO mice compared to controls. Each dot in the boxplots represents normalized pixel counts from individual bins, with colors indicating data from different mice in each group. Significance was tested using a linear mixed model with genotype as the fixed factor and individual as the random factor, nested with sections, hemisphere, and bins. n = 3 mice per genotype, with 2–3 sections from each mouse. Scale bar: 100 μm.
    Rnascope Probe Ctip2, C1 And C2, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+probe+ctip2%2C+c1+and+c2/rnascope+probe+ctip2++c1+and+c2/pmc12051487-33-0-7
    Average 90 stars, based on 1 article reviews
    rnascope probe ctip2, c1 and c2 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Cell-type-specific roles of FOXP1 in the excitatory neuronal lineage during early neocortical murine development"

    Article Title: Cell-type-specific roles of FOXP1 in the excitatory neuronal lineage during early neocortical murine development

    Journal: Cell reports

    doi: 10.1016/j.celrep.2025.115384

    (A) Violin plot demonstrating the expression levels of three representative DEGs by cell type at P0.5. Rora and Nrxn3 are downregulated whereas Kirrel3 is upregulated. (B and C) Representative RNA in situ hybridization (ISH) images for Rora (B) and Kirrel3 and Nrxn3 (C) confirm snRNA-seq findings in control and Foxp1 cKO mice at P0.5. Ctip2 was used to label L5–L6 cortical neurons. (D) The total numbers of pixels in each of the 4 bins were measured in the Ctip2 -expressing layer and normalized to the total nuclei in their respective bins. (E–G) Boxplots illustrate significant downregulation of Rora (E) and Nrxn3 (F) and upregulation of Kirrel3 (G) in the L5–L6 cortical layers of Foxp1 cKO mice compared to controls. Each dot in the boxplots represents normalized pixel counts from individual bins, with colors indicating data from different mice in each group. Significance was tested using a linear mixed model with genotype as the fixed factor and individual as the random factor, nested with sections, hemisphere, and bins. n = 3 mice per genotype, with 2–3 sections from each mouse. Scale bar: 100 μm.
    Figure Legend Snippet: (A) Violin plot demonstrating the expression levels of three representative DEGs by cell type at P0.5. Rora and Nrxn3 are downregulated whereas Kirrel3 is upregulated. (B and C) Representative RNA in situ hybridization (ISH) images for Rora (B) and Kirrel3 and Nrxn3 (C) confirm snRNA-seq findings in control and Foxp1 cKO mice at P0.5. Ctip2 was used to label L5–L6 cortical neurons. (D) The total numbers of pixels in each of the 4 bins were measured in the Ctip2 -expressing layer and normalized to the total nuclei in their respective bins. (E–G) Boxplots illustrate significant downregulation of Rora (E) and Nrxn3 (F) and upregulation of Kirrel3 (G) in the L5–L6 cortical layers of Foxp1 cKO mice compared to controls. Each dot in the boxplots represents normalized pixel counts from individual bins, with colors indicating data from different mice in each group. Significance was tested using a linear mixed model with genotype as the fixed factor and individual as the random factor, nested with sections, hemisphere, and bins. n = 3 mice per genotype, with 2–3 sections from each mouse. Scale bar: 100 μm.

    Techniques Used: Expressing, RNA In Situ Hybridization, Control

    (A and B) EdU birth-dating experiment shows that neurons generated from progenitors dividing at E15.5 remain in the DLs in Foxp1 cKOs at higher levels than in controls throughout the cortex. Some of these cells are EdU+CUX1+, indicating they have a UL cell type identity and are CTIP2−. (B) Foxp1 cKO cortices are at (top) 20× and (bottom) 63× showing EdU+CUX1+CTIP2− cells in the L5–L6 region. (C) P7.5 brains stained for EdU that was pulsed at E15.5 have significantly more cells in the DLs in Foxp1 cKOs. No significant difference was found between EdU+ cells in the ULs between genotypes. n = 5/genotype. (D) P16.5 brains from E15.5 EdU-pulsed animals with CUX1 and CTIP2 to demarcate cortical layer boundaries at 103 resolution. (E) P16.5 brains with EdU cell quantification in ULs or DLs show a significant number of EdU+ cells located in the DLs in cKOs but not controls. No significant change was seen in the number of UL EdU+ cells. n = 5/genotype. EdU+ cells were CTIP2− despite being in L5–L6, suggesting the appropriate timing of neurogenesis at E15.5 for UL cells and a likely selective migration deficit that remains at P16.5. Mann-Whitney test. Mean ± SEM shown.
    Figure Legend Snippet: (A and B) EdU birth-dating experiment shows that neurons generated from progenitors dividing at E15.5 remain in the DLs in Foxp1 cKOs at higher levels than in controls throughout the cortex. Some of these cells are EdU+CUX1+, indicating they have a UL cell type identity and are CTIP2−. (B) Foxp1 cKO cortices are at (top) 20× and (bottom) 63× showing EdU+CUX1+CTIP2− cells in the L5–L6 region. (C) P7.5 brains stained for EdU that was pulsed at E15.5 have significantly more cells in the DLs in Foxp1 cKOs. No significant difference was found between EdU+ cells in the ULs between genotypes. n = 5/genotype. (D) P16.5 brains from E15.5 EdU-pulsed animals with CUX1 and CTIP2 to demarcate cortical layer boundaries at 103 resolution. (E) P16.5 brains with EdU cell quantification in ULs or DLs show a significant number of EdU+ cells located in the DLs in cKOs but not controls. No significant change was seen in the number of UL EdU+ cells. n = 5/genotype. EdU+ cells were CTIP2− despite being in L5–L6, suggesting the appropriate timing of neurogenesis at E15.5 for UL cells and a likely selective migration deficit that remains at P16.5. Mann-Whitney test. Mean ± SEM shown.

    Techniques Used: Generated, Staining, Migration, MANN-WHITNEY


    Figure Legend Snippet:

    Techniques Used: Recombinant, Electron Microscopy, RNAscope, Isolation, Software, Microscopy

    Related Articles

    other:

    Article Title: Cell-type-specific roles of FOXP1 in the excitatory neuronal lineage during early neocortical murine development.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Tapestation HS1000 Deposited data Raw and Analyzed Data This paper GEO: GSE267673 (GSE267670, GSE267671) Experimental models: Organisms/strains Mouse: Foxp1 flox/flox Dr. Haley Tucker, University of Texas (Feng et al.33) Strain#: 017699; RRID: IMSR_JAX:017699 Mouse: Emx1-Cre Jackson Laboratory Strain#: 005628; RRID: IMSR_JAX:005628 Oligonucleotides Foxp1-flox Forward genotyping primers: Usui et al.30 N/A F-50-CCAGGGATCAGAGATTACT GTAGC-30 Foxp1-flox Reverse genotyping primers: Usui et al.30 N/A R-50-CACCCTCTCCAAGTCTGCCTCAG-30 Emx1 WT Forward genotyping primers: Usui et al.30 N/A F-50-AAGGTGTGGTTCCAGAATCG-30 Emx1 WT Reverse genotyping primers: Usui et al.30 N/A R-50-CTCTCCACCAGAAGGCTGAG-30 Emx1 KO Forward genotyping primers: Usui et al.30 N/A F-50-GCGGTCTGGCAGTAAAAACTATC-30 Emx1 KO Reverse genotyping primers: Usui et al.30 N/A R-50-GTGAAACAGCATTGCTGTCACTT-30 Software and algorithms CellRanger v2.1.1 (mkref, mkfastq, count) 10X Genomics; Zheng et al.82 https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/3.

    RNAscope:

    Article Title: Cell-type-specific roles of FOXP1 in the excitatory neuronal lineage during early neocortical murine development
    Article Snippet: Normal Donkey Serum , Millipore Sigma , Cat# S30-100ML. .. RNAscope probe Ctip2, C1 and C2 , ACD Bio-techne , Cat# 413271. .. RNAscope probe Rora, C1 , ACD Bio-techne , Cat# 520031.



    Similar Products

    rnascope probe ctip2, c1 and c2  (Advanced Cell Diagnostics Inc)
    90
    Advanced Cell Diagnostics Inc rnascope probe ctip2, c1 and c2
    (A) Violin plot demonstrating the expression levels of three representative DEGs by cell type at P0.5. Rora and Nrxn3 are downregulated whereas Kirrel3 is upregulated. (B and C) Representative RNA in situ hybridization (ISH) images for Rora (B) and Kirrel3 and Nrxn3 (C) confirm snRNA-seq findings in control and Foxp1 cKO mice at P0.5. <t>Ctip2</t> was used to label L5–L6 cortical neurons. (D) The total numbers of pixels in each of the 4 bins were measured in the Ctip2 -expressing layer and normalized to the total nuclei in their respective bins. (E–G) Boxplots illustrate significant downregulation of Rora (E) and Nrxn3 (F) and upregulation of Kirrel3 (G) in the L5–L6 cortical layers of Foxp1 cKO mice compared to controls. Each dot in the boxplots represents normalized pixel counts from individual bins, with colors indicating data from different mice in each group. Significance was tested using a linear mixed model with genotype as the fixed factor and individual as the random factor, nested with sections, hemisphere, and bins. n = 3 mice per genotype, with 2–3 sections from each mouse. Scale bar: 100 μm.
    Rnascope Probe Ctip2, C1 And C2, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+probe+ctip2%2C+c1+and+c2/rnascope+probe+ctip2++c1+and+c2/pmc12051487-33-0-7
    Average 90 stars, based on 1 article reviews
    rnascope probe ctip2, c1 and c2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Violin plot demonstrating the expression levels of three representative DEGs by cell type at P0.5. Rora and Nrxn3 are downregulated whereas Kirrel3 is upregulated. (B and C) Representative RNA in situ hybridization (ISH) images for Rora (B) and Kirrel3 and Nrxn3 (C) confirm snRNA-seq findings in control and Foxp1 cKO mice at P0.5. Ctip2 was used to label L5–L6 cortical neurons. (D) The total numbers of pixels in each of the 4 bins were measured in the Ctip2 -expressing layer and normalized to the total nuclei in their respective bins. (E–G) Boxplots illustrate significant downregulation of Rora (E) and Nrxn3 (F) and upregulation of Kirrel3 (G) in the L5–L6 cortical layers of Foxp1 cKO mice compared to controls. Each dot in the boxplots represents normalized pixel counts from individual bins, with colors indicating data from different mice in each group. Significance was tested using a linear mixed model with genotype as the fixed factor and individual as the random factor, nested with sections, hemisphere, and bins. n = 3 mice per genotype, with 2–3 sections from each mouse. Scale bar: 100 μm.

    Journal: Cell reports

    Article Title: Cell-type-specific roles of FOXP1 in the excitatory neuronal lineage during early neocortical murine development

    doi: 10.1016/j.celrep.2025.115384

    Figure Lengend Snippet: (A) Violin plot demonstrating the expression levels of three representative DEGs by cell type at P0.5. Rora and Nrxn3 are downregulated whereas Kirrel3 is upregulated. (B and C) Representative RNA in situ hybridization (ISH) images for Rora (B) and Kirrel3 and Nrxn3 (C) confirm snRNA-seq findings in control and Foxp1 cKO mice at P0.5. Ctip2 was used to label L5–L6 cortical neurons. (D) The total numbers of pixels in each of the 4 bins were measured in the Ctip2 -expressing layer and normalized to the total nuclei in their respective bins. (E–G) Boxplots illustrate significant downregulation of Rora (E) and Nrxn3 (F) and upregulation of Kirrel3 (G) in the L5–L6 cortical layers of Foxp1 cKO mice compared to controls. Each dot in the boxplots represents normalized pixel counts from individual bins, with colors indicating data from different mice in each group. Significance was tested using a linear mixed model with genotype as the fixed factor and individual as the random factor, nested with sections, hemisphere, and bins. n = 3 mice per genotype, with 2–3 sections from each mouse. Scale bar: 100 μm.

    Article Snippet: RNAscope probe Ctip2, C1 and C2 , ACD Bio-techne , Cat# 413271.

    Techniques: Expressing, RNA In Situ Hybridization, Control

    (A and B) EdU birth-dating experiment shows that neurons generated from progenitors dividing at E15.5 remain in the DLs in Foxp1 cKOs at higher levels than in controls throughout the cortex. Some of these cells are EdU+CUX1+, indicating they have a UL cell type identity and are CTIP2−. (B) Foxp1 cKO cortices are at (top) 20× and (bottom) 63× showing EdU+CUX1+CTIP2− cells in the L5–L6 region. (C) P7.5 brains stained for EdU that was pulsed at E15.5 have significantly more cells in the DLs in Foxp1 cKOs. No significant difference was found between EdU+ cells in the ULs between genotypes. n = 5/genotype. (D) P16.5 brains from E15.5 EdU-pulsed animals with CUX1 and CTIP2 to demarcate cortical layer boundaries at 103 resolution. (E) P16.5 brains with EdU cell quantification in ULs or DLs show a significant number of EdU+ cells located in the DLs in cKOs but not controls. No significant change was seen in the number of UL EdU+ cells. n = 5/genotype. EdU+ cells were CTIP2− despite being in L5–L6, suggesting the appropriate timing of neurogenesis at E15.5 for UL cells and a likely selective migration deficit that remains at P16.5. Mann-Whitney test. Mean ± SEM shown.

    Journal: Cell reports

    Article Title: Cell-type-specific roles of FOXP1 in the excitatory neuronal lineage during early neocortical murine development

    doi: 10.1016/j.celrep.2025.115384

    Figure Lengend Snippet: (A and B) EdU birth-dating experiment shows that neurons generated from progenitors dividing at E15.5 remain in the DLs in Foxp1 cKOs at higher levels than in controls throughout the cortex. Some of these cells are EdU+CUX1+, indicating they have a UL cell type identity and are CTIP2−. (B) Foxp1 cKO cortices are at (top) 20× and (bottom) 63× showing EdU+CUX1+CTIP2− cells in the L5–L6 region. (C) P7.5 brains stained for EdU that was pulsed at E15.5 have significantly more cells in the DLs in Foxp1 cKOs. No significant difference was found between EdU+ cells in the ULs between genotypes. n = 5/genotype. (D) P16.5 brains from E15.5 EdU-pulsed animals with CUX1 and CTIP2 to demarcate cortical layer boundaries at 103 resolution. (E) P16.5 brains with EdU cell quantification in ULs or DLs show a significant number of EdU+ cells located in the DLs in cKOs but not controls. No significant change was seen in the number of UL EdU+ cells. n = 5/genotype. EdU+ cells were CTIP2− despite being in L5–L6, suggesting the appropriate timing of neurogenesis at E15.5 for UL cells and a likely selective migration deficit that remains at P16.5. Mann-Whitney test. Mean ± SEM shown.

    Article Snippet: RNAscope probe Ctip2, C1 and C2 , ACD Bio-techne , Cat# 413271.

    Techniques: Generated, Staining, Migration, MANN-WHITNEY

    Journal: Cell reports

    Article Title: Cell-type-specific roles of FOXP1 in the excitatory neuronal lineage during early neocortical murine development

    doi: 10.1016/j.celrep.2025.115384

    Figure Lengend Snippet:

    Article Snippet: RNAscope probe Ctip2, C1 and C2 , ACD Bio-techne , Cat# 413271.

    Techniques: Recombinant, Electron Microscopy, RNAscope, Isolation, Software, Microscopy